Yes, you can assign function keys (F1-F12) to PlayIt Live actions through the keyboard settings. Go to File > Settings, click the Keyboard tab, select an action from the list, then click the key field and press your desired function key. To make shortcuts work even when PlayIt Live doesn't have focus (useful with Stream Decks or other controllers), tick the Global trigger checkbox at the bottom of the keyboard settings.

When using global triggers, it's important to use modified keys (combining function keys with Ctrl, Alt, or Shift) rather than unmodified function keys on their own. For example, use Ctrl+F1 instead of just F1. This prevents unintended triggers in PlayIt Live when you're using other software. Many applications use F1 for Help menus, F5 for refresh, and other standard shortcuts. If you assign an unmodified function key with global trigger enabled, pressing that key in another application will trigger the action in both programs simultaneously - the other software will perform its function whilst PlayIt Live also responds to the keypress, potentially causing unexpected behaviour during your broadcast. For critical playback controls, consider using more accessible combinations like Ctrl+Arrow keys that are easier to reach and less likely to cause unintended triggers.